THE MILESCU LABs

Fine science since 2011...

 

Our lab is interested in how ion channels function at the molecular level and how they contribute to neuronal firing mechanisms.


At the molecular level, we are focused on the mechanisms underlying voltage sensing, and on the interactions between ion channels and the cellular lipid membrane. We use site-directed mutagenesis and electrophysiological techniques on Xenopus oocytes to understand how slight changes in protein structure affect the molecular function of the ion channel.

We study ion channels and neuronal firing mechanisms in rodent brain slices and in acutely dissociated neurons. In particular, we are interested in the neuronal circuitry responsible for the generation of respiratory rhythm. Using an in vitro brainstem slice preparation, we investigate how voltage-gated ion channels enable individual neurons to function in a specific way, and how these neurons interact in a network to produce different patterns of activity. We use a combination of electrophysiology (patch-clamp), imaging (multiphoton laser scanning microscopy), and real-time computation (dynamic clamp) to record and analyze the ionic currents passing through ion channels, and the firing activities of individual neurons or circuits.

A new release of QuB is now available, featuring 3D data mapping and experiment control and visualization.